Dideoxynucleosides are modified nucleosides that lack the 3’-OH group on their sugar moiety, which is required for the formation of the phosphodiester bond that links nucleotides in a growing DNA strand. Because of this, dideoxynucleosides act as chain-terminators during DNA synthesis.
DNA Sequencing: HUAREN's Dideoxynucleosides are essential for Sanger sequencing, enabling precise DNA sequence determination by terminating DNA synthesis.
PCR: The Dideoxynucleosides are used in chain termination PCR for mutation detection and genotyping.
Antiviral Research: Inhibits viral DNA polymerases, aiding in the development of antiviral drugs.
Cancer Research: The Dideoxynucleosides block DNA synthesis in cancer cells, useful for therapeutic studies.
Site-Directed Mutagenesis: Facilitates specific DNA sequence changes to study gene function.
Enzyme Kinetics: The Dideoxynucleosides are used in assays to study DNA polymerase activity.
Synthetic Biology: Helps in creating artificial genes and genetic circuits.